NAME: TARNOPOLSKY, MARK A., M.D., Ph.D., FABEM, F.R.C.P.(C) Associate Professor DEPARTMENT: Medicine E-MAIL ADDRESS : tarnopol@mcmaster.ca

1. The overall theme of our research is an evaluation of treatment strategies to alternate the effect of neuromuscular disorders and again on skeletal muscle function.
   
   
2. Currently, we are looking at a number of nutritional and exercise interventions to enhance muscle function in response to neuromuscular, neurometabolic disorders as well as in the aging process. We have transgenic animal models of several neuromuscular disorders and are also completing clinical trials in patients with these conditions. Another line of research is the role of mitochondria in aging and the role of oxidative stress/apoptosis in skeletal muscle damage in response to exercise and neuromuscular disorders.
   
   
3. We use a variety of methods in the laboratory from strength and functional outcome measures in the dry laboratory to Western blotting, RT-PCR, PCR, enzyme analysis, metabolite analysis, ELISA, microscopy (immuno, EM and fluorescent methods), GC-MS, micro array, HPLC and RIA.

PUBLICATIONS

• Mahoney DJ, Carey K, Fu MH, Snow R, Smith DC, Parise G, Tarnopolsky M. (2004) Real-time RT-PCR analysis of housekeeping genes in human skeletal muscle following acute exercise. Physiol. Genomics 18:226-231.
  
  
• Mahoney, DJ, Parise, G, Melov, S, Safdar, A, Tarnopolsky, MA. (2005) Analysis of global mRNA expression in human skeletal muscle during recovery from endurance exercise. FASEB (in press).
  
  
• Hamadeh M, Devries M, Tarnopolsky M. (2005) Estrogen supplementation reduces whole body leucine and carbohydrate oxidation and increases lipid oxidation in men during endurance exercise. J. of Clin. Endocrinology & Metabolism, epub ahead of print.
  
  
• Kaczor J, Ziolkowski W, Popinigis J, Tarnopolsky M. (2005) Anaerobic and aerobic enzyme activities in human skeletal muscle from children and young adults. Pediatric Research 57(3): 331-335.
  
  
• Parise G, Brose A, Tarnopolsky M. (2005) Resistance exercise training decreases oxidative damage to DNA and increases cytochrome oxidase activity in older adults. Experimental Gerontology 40:173-180.

KEY WORDS

Mitochondria, Estradiol, Muscle Damage, Apoptosis, Inflammation, Skeletal, Muscle, Exercise, Neuromuscular Disorders, Aging, Corticosteroids