Clinical Immunology & Allergy Residency Program

Rotation-Specific Goals and Objectives for Laboratory Rotation

The overall objective of this period of training is to provide the resident with the necessary skills, competence and confidence to be aware of the issues that occur in biochemical laboratory analysis that may impact on the utility of laboratory results.

Operation of automated immunoassay analyzers:

  1. Understand the principles of the operation of automated analyzers
  2. Interpretation of results generated

Basic laboratory techniques and centrifugation:

  1. Methods of standardization and calibration
  2. Identification of common method interferences
  3. To detect errors and sources of error

Knowledge of the terminology used to evaluate an Analytical Method:

  • Bias
  • Imprecision
  • Sensitivity
  • Specificity
  • Investigation of common interferences
  • Range
  • Criteria for acceptability

Describe the laboratory techniques used in Clinical Immunology and Allergy, including determination of normal values, indications and limitations, availability:

  1. Flow cytometry (cell surface markers & receptors)
    • lymphocyte enumeration (immunophenotyping)
    • cytokines
    • dihydro-rhodamine oxidation
    • total and specific-IgE
    • ELISA (EIA)
    • Bioplex ANA
    • AntiTTG (IgA)
    • Thyroid peroxidase (replacing thyroglobulin and microsomal Abs)
    • Indirect Immunofluorescent Assay (IFA)
    • ANCA
    • Mitochondrial AB, Smooth muscle Ab, Parietal cell Ab, Endomysial Ab
    • Nephelometry
    • Rheumatoid factor
    • Radial immunodiffusion (quantitative precipitin reaction)
    • C1 Inhibitor
  2. CH50
  3. Isohemagglutinins
  4. Functional Antibodies
    • Serum IgG to tetanus, diphtheria
    • Measles, mumps, rubella serology
  5. Precipitating IgG antibodies (Precipitins)
  6. Functional Lymphocyte Assay (sent out)
    • Mitogen proliferation
    • Antigen proliferation
  7. Immunoglobulin levels
  8. Complement assays (C3, C4, CH100)
  9. Immune complex assays (e.g. C1q binding)

Demonstrate an ability to listen effectively to laboratory staff, colleagues and patients and their families.

Describe the utility of diagnostic tests used in Clinical Immunology Allergy to balance costs and availability in optimizing patient care

Exhibit appropriate personal and interpersonal professional behaviours