McMaster University

McMaster University

Bryan J. Clarke

, PhD

Associate Professor
Pathology and Molecular Medicine

Associate Member, Biology
Area Coordinator, Hemostasis, Thromboembolism & Atherosclerosis
Medical Sciences Graduate Program

McMaster University
4N65 Health Sciences Centre
905-525-9140 ext. 22372
bjclarke@mcmaster.ca

Assistant: Arlene Scopaz

Bryan J. Clarke

Faculty Biography

Education and Professional Standing

PhD, Anatomy, University of Toronto, 1975
MSc, Microbiology, University of Manitoba, 1970
BSc, Agriculture, McGill University, 1967

Interests

Investigation of normal and abnormal coagulation in man and animals. Our principal focus is on initiation of coagulation via the extrinsic pathway, i.e. the interaction of plasma factor VII(FVII) and its cell surface receptor tissue factor (TF).

We are currently involved in three areas of research: (a) elucidation of the amino acid residues of human FVII which are directly involved in the binding to TF; (b) analysis of the role of the FVII-TF complex in disseminated intravascular coagulation in the rabbit; and (c) study of the effect of FVII and other coagulation proteins on the growth of tumours in the rabbit lung.

Our lab utilizes a variety of techniques to attempt to address fundamental questions in coagulation. These methods include cell culture, recombinant DNA techniques and immunological methods. We also have the capability to perform computer-assisted molecular modelling (in collaboration with Dr. Daniel Yang of the Biochemistry Department).


Selected Publications

  • Clarke BJ, Sridhara S, Woskowska Z, Blajchman MA. Consumption of plasma factor VII in a rabbit model of non-overt disseminated intravascular coagulation. Thromb Res 108:329-334, 2002.
  • Leonard BJN, Clarke BJ, Sridhara S, Kelley R, Ofosu FA, Blajchman MA. Activation and active site occupation alter conformation in the region of the first epidermal growth factor-like domain of human factor VII. J Biol Chem 275:34894-34900, 2000.
  • Leonard, B., Chen, Q., Blajchman, M.A., Ofosu, F.A., Sridhara, S., Yang, D. and Clarke, B.J. (1998) Factor VII deficiency caused by a structural variant N57D of the first epidermal growth factor domain. Blood 91:142.
  • Sridhara, S., Chaing, S., High, K.A., Blajchman, M.A. and Clarke, B.J. (1996) Activation of a recombinant human factor VII structural analogue alters its affinity of binding to tissue factor. Amer. J. Hematol. 53:66.
  • Clarke, B.J. and Sridhara, S. (1996) Incomplete gamma carboxylation of human coagulation factor VII. Differential effects on tissue factor binding and enzymatic activity. Br. J. Hematol. 93:445.
  • Chaing, S., Clarke, B.J., Sridhara, S., Chu, K., Friedman, P., Van Dusen, W., Roberts, H.R., Blajchman, M., Monroe, D. and High, K.A. (1994) Severe factor VII deficiency due to mutations abolishing the cleavage site for activation and altering binding to tissue factor. Blood 83:3524.
  • Sridhara, S., Clarke, B.J., Ofosu, F.A., High, K.A. and Blajchman, M.A. (1993) The direct binding of factor VII in human plasma to recombinant human tissue factor. Thromb. Res. 70:307.
  • Brothers A, Clarke, B.J., Sheffield, W.P. and Blajchman, M.A. (1993) Cloning and sequencing of rabbit factor VII cDNA. Thromb. Res. 69:231.
  • Clarke, B.J., Ofosu, F.A., Sridhara, S., Bona, R.D., Rickles, F.R. and Blajchman, M.A. (1992) The first epidermal growth factor-like domain of human coagulation factor VII is essential for complex formation with tissue factor. FEBS Lett. 298:206.
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