Johannes Zeidler

, PhD

Assistant Professor
Pathology and Molecular Medicine

Division: Clinical Pathology

Specialty: Toxicology

Hamilton General Hospital
Clinical Chemistry/Immunology Laboratory
S1 room 1-02
905-527-4322 ext 76267
jzeidler@hhsc.ca

Education and Professional Standing

• DABCC, Toxicological Chemistry, 2007
• PhD, Plant Physiology, University of Karlsruhe (Germany), 2001
• Diploma (equiv. MSc), Organic Chemistry, University of Karlsruhe (Germany), 1994

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Interests

Research Focus

As a bioorganic, analytical, synthetic and toxicological chemist I develop new assays for small molecules using liquid or gas chromatography, mass spectrometry and other techniques to support service and collaborative research. Molecules of interest are drugs and metabolites and any other endogenous or exogenous compounds that can be analysed with the following techniques: Liquid chromatography with UV, fluorescence, electrochemical or triple quadrupole mass spectrometry detection; gas chromatography with flame ionisation, thermionic sensitive and mass spectrometric detection. Available assays include e.g. cotinine and 25-hydroxyvitamin D in plasma or serum by liquid chromatography tandem mass spectrometry (LC-MS/MS).

Clinical Focus

I am responsible for the special chemistry/toxicology laboratory in the Hamilton Laboratory Medicine Program (HRLMP) I advise on laboratory issues around therapeutic drug monitoring and clinical toxicology.

Academic Interests

I am involved in teaching in the undergraduate and postgraduate programs of the department.

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Selected Publications

• Sohn, K.-Y., Zeidler, J.: Deuterium labelled Sirolimus: Synthesis and Assessment as Internal Standard in a New LC/MS/MS method. - Poster presentation at conference "Mass Spectrometry: Applications to the Clinical Laboratory 2010 (MSACL 2010)”, Feb 6-10, San Diego.
• Huang, Y., Zeidler, J.: A Fast Measurement of Clozapine by Liquid-Chromatography Tandem Mass Spectrometry. – Poster E-105 at AACC Conference 2009, July 19-23, Chicago, Clin. Chem.  55, Suppl. (2009).
• Oremus M, Zeidler J, Ensom MHH, Matsuda-Abedini M, Balion C, Booker L, Archer C, Raina P: Evidence Report on the Utility of Monitoring Mycophenolic Acid in Solid Organ Transplant Patients. Evidence Report/ Technology Assessment No. 164. (Prepared by the McMaster University Evidence-based Practice Center, under Contract No. 290-02-0020.) AHRQ Publication No.08-E006) Rockville, MD: Agency for Healthcare Research and Quality (2008).
• Zeidler J, Gauthier D: APCI LC-MS/MS analysis of a polar base using normal phase chromatography on a fluorinated column: Quantitation of cotinine in plasma. - Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied Topics June 5-10, 2005 San Antonio, Texas (2005).
• Zeidler J, Sayer BG, Spenser ID: Biosynthesis of vitamin B1 in yeast. Derivation of the pyrimidine unit from pyridoxine and histidine. Intermediacy of urocanic acid. – J. Am. Chem. Soc. 125, 13094-13105 (2003).
• Zeidler J, Ullah N, Gupta RN, Pauloski RM, Sayer BG, Spenser ID: 2’-Hydroxypyridoxol, a biosynthetic precursor of vitamins B6 and B1 in yeast. – J. Am. Chem. Soc. 124, 4542-4543 (2002).
• Jomaa H, Wiesner J, Sanderbrand S, Altincicek B, Weidemeyer C, Hintz M, Türbachova I, Eberl M, Zeidler J, Lichtenthaler HK, Soldati D, Beck E: Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs. - Science 285, 1573-1576 (1999).
• Zeidler JG, Lichtenthaler HK, May HU, Lichtenthaler FW: Is isoprene emitted by plants synthesized via the novel isopentenyl pyrophosphate pathway ?. - Z. Naturforsch. 52c, 15-23 (1997).